ZEBRAFISH RETINITIS PIGMENTOSA RETINA SINGLE-CELL
Zebrafish provides an excellent model system to investigate the molecular mechanisms involved in the development and regeneration of the retina. As a step toward investigating regenerative therapies, we developed a zebrafish model of Retinitis Pigmentosa that displays continuous rod photoreceptor degeneration and regeneration. This model expresses a transgene coding for mouse rhodopsin carrying the P23H mutation and an epitope tag in rod photoreceptors driven by the zebrafish rhodopsin promoter (1). At this site, you can explore single-cell transcriptome data from retinas of adult wild-type Zebrafish and the P23H rhodopsin transgenic model of RP. We provide a benchmark atlas of retinal cell type transcriptomes in Zebrafish and find changes in all retinal cell types in the P23H model (2).
Single cell transcriptome from adult Zebrafish retina (WT) (10x Genomics, Chromium Next GEM Single Cell 3’ v3.1)
View Dataset
Single cell transcriptome from adult Zebrafish retina –integrated dataset - WT and P23H RP model (10x Genomics, Chromium Next GEM Single Cell 3’ v3.1)
View DatasetReferences
- Santhanam, A.; Shihabeddin, E.; Atkinson, J.A.; Nguyen, D.; Lin, Y.-P.; O’Brien, J. A Zebrafish Model of Retinitis Pigmentosa Shows Continuous Degeneration and Regeneration of Rod Photoreceptors. Cells 2020, 9, 2242.
- Abirami Santhanam, Eyad Shihabeddin, Haichao Wei, Jiaqian Wu, John O'Brien. Transcriptomic remodeling of the retina in a Zebrafish model of Retinitis Pigmentosa. bioRxiv 2022.10.04.510882; doi: https://doi.org/10.1101/2022.10.04.510882